Probing eukaryotic genome functions with synthetic chromosomes

Zhouqing Luo; Stefan A Hoffmann; Shuangying Jiang; Yizhi Cai; Junbiao Dai

doi:10.1016/j.yexcr.2020.111936

Probing eukaryotic genome functions with synthetic chromosomes

Exp Cell Res. 2020 May 1;390(1):111936. doi: 10.1016/j.yexcr.2020.111936. Epub 2020 Mar 9.

Authors

Zhouqing Luo¹, Stefan A Hoffmann², Shuangying Jiang¹, Yizhi Cai³, Junbiao Dai⁴

Affiliations

¹ Guangdong Provincial Key Laboratory of Synthetic Genomics, Shenzhen Key Laboratory of Synthetic Genomics, Center for Synthetic Genomics, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
² Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK.
³ Guangdong Provincial Key Laboratory of Synthetic Genomics, Shenzhen Key Laboratory of Synthetic Genomics, Center for Synthetic Genomics, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China; Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK. Electronic address: [email protected].
⁴ Guangdong Provincial Key Laboratory of Synthetic Genomics, Shenzhen Key Laboratory of Synthetic Genomics, Center for Synthetic Genomics, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China; College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518055, China. Electronic address: [email protected].

PMID: 32165165
DOI: 10.1016/j.yexcr.2020.111936

Abstract

The ability to redesign and reconstruct a cell at whole-genome level provides new platforms for biological study. The international synthetic yeast genome project-Sc2.0, designed by interrogating knowledge amassed by the yeast community to date, exemplifies how a classical synthetic biology "design-build-test-learn" engineering cycle can effectively test hypotheses about various genome fundamentals. The genome reshuffling SCRaMbLE system implemented in synthetic yeast strains also provides unprecedented diversified resources for genotype-phenotype study and yeast metabolic engineering. Further development of genome synthesis technology will shed new lights on complex biological processes in higher eukaryotes.

Keywords: Genotype-phenotype relationship; Metabolic engineering; SCRaMbLE; Synthetic chromosome; Yeast genome.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Chromosomes, Artificial, Yeast / genetics*
Genetic Engineering / methods*
Genome, Fungal*
Genomics / methods
Saccharomyces cerevisiae

Grants and funding

BB/P02114X/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom