Preparation of As4S4/Fe3O4 nanosuspensions and in-vitro verification of their anticancer activity

Mater Sci Eng C Mater Biol Appl. 2020 May:110:110683. doi: 10.1016/j.msec.2020.110683. Epub 2020 Jan 20.

Abstract

Multifunctional nanoparticulate systems, especially those used in medicine, are currently of great interest. In this work, the in-vitro anticancer activity of As4S4/Fe3O4 composites dispersed in a water solution of Poloxamer 407 on breast MCF-7 and tongue SCC-25 cancer cells was verified. An increase in apoptotic cells as a consequence of higher caspase activities, a decrease in mitochondrial membrane potential and an accumulation of cells in the G2/M and subG0/G1 phases were detected after treatment with the As4S4/Fe3O4 nanosuspensions. The sterically stabilized nanosuspensions were characterized in relation to their particle size distribution, zeta potential and long-term stability properties. The interaction between the solid and liquid phases of the nanosuspensions was also studied using Fourier transform infrared spectroscopy.

Keywords: Anticancer effect; Magnetite; Mechanochemistry; Multifunctional nanoparticles; Realgar.

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Arsenicals / pharmacology*
  • Caspases / metabolism
  • Cell Cycle / drug effects
  • Cell Line, Tumor
  • Ferric Compounds / pharmacology*
  • Humans
  • Magnetic Phenomena
  • Membrane Potential, Mitochondrial / drug effects
  • Nanocomposites / chemistry
  • Nanocomposites / ultrastructure
  • Nanoparticles / chemistry*
  • Particle Size
  • Reproducibility of Results
  • Spectroscopy, Fourier Transform Infrared
  • Static Electricity
  • Sulfides / pharmacology*
  • Suspensions / chemistry*
  • Temperature

Substances

  • Antineoplastic Agents
  • Arsenicals
  • Ferric Compounds
  • Sulfides
  • Suspensions
  • ferric oxide
  • Caspases
  • tetraarsenic tetrasulfide