Abstract
RNA demethylase has recently been known to be associated with cancer development but its selective inhibitors as anti-cancer agents have rarely been investigated to date. Herein, we have developed a fluorescent nanobiosensor which enables efficient quantitative analysis of RNA demethylase ALKBH5 activity and shows a high potential for robust inhibitor screening.
MeSH terms
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AlkB Homolog 5, RNA Demethylase / chemistry*
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Biosensing Techniques*
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DNA-Binding Proteins / chemistry*
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Demethylation
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Endoribonucleases / chemistry*
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Escherichia coli Proteins / chemistry*
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Graphite / chemistry*
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Nanoparticles / chemistry*
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Spectrometry, Fluorescence
Substances
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DNA-Binding Proteins
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Escherichia coli Proteins
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MazF protein, E coli
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graphene oxide
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Graphite
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AlkB Homolog 5, RNA Demethylase
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Endoribonucleases