Persistence of Intraluminal Thrombus Makes Saccular Aneurysm More Biologically Active than Fusiform in an Experimental Rat Model

J Vasc Res. 2020;57(3):164-176. doi: 10.1159/000506159. Epub 2020 Mar 27.

Abstract

Introduction: Saccular aneurysms are thought to have a worse prognosis than fusiform aneurysms in humans, due to hemodynamic reasons. However, data comparing hemodynamic and biology in saccular and fusiform aneurysms are lacking. The main objective was to evaluate the impact of aneurysm morphology on intra-luminal thrombus (ILT) formation and activity.

Methods: Forty Lewis rats were ran-domly divided into 2 groups of 20: "saccular" (Group A) and "fusiform" (Group B) aneurysms. Decellularized thoracic aortas from guinea pigs were xenografted to create saccular or fusiform aneurysms. Final imaging evaluation of the aneurysms was carried out during the third week, by quantitative Doppler ultrasound and magnetic resonance imaging. Assays of myeloperoxidase (MPO), platelet factor 4 (PF4), advanced oxidation protein products (AOPPs) iron and matrix metallopeptidase-9 (MMP-9) were performed as biological criteria.

Results: Quantitatively, saccular aneurysms are characterized by a more thicker ILT, lower inflow velocities and more important relative backflow velocities as compared to fusiform aneurysms. Compared to fusiform, saccular aneurysms released significantly more MPO (p = 0.004), PF4 (p = 0.02), AOPPs (p < 0.002), iron (p < 0.0001) and MMP-9 (p < 0.04).

Conclusion: Experimental saccular and fusiform aneurysms show differential specific hemodynamics, which seem to impact the histology and the biology of the ILT in each type of aneurysm.

Keywords: Abdominal aortic aneurysm; Doppler ultrasound; Hemodynamic; Magnetic resonance imaging; Matrix metallopeptidase-9; Myeloperoxidase.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Advanced Oxidation Protein Products / metabolism
  • Animals
  • Aorta, Abdominal / diagnostic imaging
  • Aorta, Abdominal / metabolism
  • Aorta, Abdominal / physiopathology*
  • Aortic Aneurysm, Abdominal / diagnostic imaging
  • Aortic Aneurysm, Abdominal / metabolism
  • Aortic Aneurysm, Abdominal / physiopathology*
  • Disease Models, Animal
  • Guinea Pigs
  • Hemodynamics*
  • Iron / metabolism
  • Male
  • Matrix Metalloproteinase 9 / metabolism
  • Peroxidase / metabolism
  • Platelet Factor 4 / metabolism
  • Rats, Inbred Lew
  • Thrombosis / diagnostic imaging
  • Thrombosis / metabolism
  • Thrombosis / physiopathology*
  • Time Factors

Substances

  • Advanced Oxidation Protein Products
  • Platelet Factor 4
  • Iron
  • Peroxidase
  • Matrix Metalloproteinase 9
  • Mmp9 protein, rat