Abstract
Various types of ADP-ribosyl protein conjugates were synthesized and their chemical stability was compared with that of cysteine-linked ADP-ribosyl groups as formed by incubation of transducin or Gi/Go proteins with NAD and pertussis toxin. Treatment with 0.1 mM HgCl2 specifically cleaved the cysteine-linked conjugates. This may provide a tool for the quantitation of modified Gi/Go proteins as well as of other acceptors modified by ADP-ribose at cysteine residues in the presence of other ADP-ribosyl proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Diphosphate Ribose / metabolism*
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Animals
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Cattle
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Cysteine
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Histones / metabolism*
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Hydrolysis
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Mercury / pharmacology*
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Mitochondria, Heart / metabolism
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NAD / metabolism
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Peptide Elongation Factor 2
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Peptide Elongation Factors / metabolism
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Peptides / metabolism*
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Pertussis Toxin*
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Proteins / metabolism*
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Submitochondrial Particles / metabolism
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Transducin / metabolism
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Virulence Factors, Bordetella / metabolism*
Substances
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Histones
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Peptide Elongation Factor 2
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Peptide Elongation Factors
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Peptides
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Proteins
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Virulence Factors, Bordetella
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NAD
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Adenosine Diphosphate Ribose
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Pertussis Toxin
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Transducin
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Mercury
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Cysteine