Graphene-polydopamine-C60 nanohybrid: an efficient protective agent for NO-induced cytotoxicity in rat pheochromocytoma cells

Zhen Hu; Yudong Huang; Chunhua Zhang; Li Liu; Jun Li; Yafei Wang

doi:10.1039/c4tb01446d

Graphene-polydopamine-C₆₀ nanohybrid: an efficient protective agent for NO-induced cytotoxicity in rat pheochromocytoma cells

J Mater Chem B. 2014 Dec 28;2(48):8587-8597. doi: 10.1039/c4tb01446d. Epub 2014 Oct 31.

Authors

Zhen Hu¹, Yudong Huang, Chunhua Zhang, Li Liu, Jun Li, Yafei Wang

Affiliation

¹ School of Chemical Engineering and Technology, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150001, China. [email protected] [email protected].

PMID: 32262217
DOI: 10.1039/c4tb01446d

Abstract

Here we have designed and developed a facile and mild synthetic approach to prepare a graphene-polydopamine-C₆₀ nanohybrid for biological applications. Graphene oxide (GO) is surface modified and reduced via polydopamine (PDA) chemistry. Using nucleophilic addition, the rGO-PDA-C₆₀ nanohybrid is then prepared. Benefiting from the unique nanostructure, the versatile hybrid of rGO-PDA-C₆₀ limits the aggregation of C₆₀ and enhances the cell uptake, which displays synergistic protective effects on the NO-induced cytotoxicity. Investigation of the possible NO-scavenging activities of rGO-PDA-C₆₀ demonstrates that it expresses direct scavenging activity toward NO both under cell and cell free conditions. As expected, rGO-PDA-C₆₀ prevents NO-induced cytotoxicity and blocks the following apoptosis events, such as lipid peroxidation, mitochondrial depolarization, and caspase-3 activation. The study presents the potential of nanocarbons for preventing the NO-mediated cell injury.