Simultaneous measurement of biochemical phenotypes and gene expression in single cells

Amanda L Richer; Kent A Riemondy; Lakotah Hardie; Jay R Hesselberth

doi:10.1093/nar/gkaa240

Simultaneous measurement of biochemical phenotypes and gene expression in single cells

Nucleic Acids Res. 2020 Jun 4;48(10):e59. doi: 10.1093/nar/gkaa240.

Authors

Amanda L Richer^{1

2}, Kent A Riemondy³, Lakotah Hardie¹, Jay R Hesselberth^{1

2

3}

Affiliations

¹ Department of Biochemistry and Molecular Genetics, Aurora, CO 80045, USA.
² Molecular Biology Program.
³ RNA Bioscience Initiative, University of Colorado School of Medicine, Aurora, CO 80045, USA.

Abstract

Methods to measure heterogeneity among cells are rapidly transforming our understanding of biology but are currently limited to molecular abundance measurements. We developed an approach to simultaneously measure biochemical activities and mRNA abundance in single cells to understand the heterogeneity of DNA repair activities across thousands of human lymphocytes, identifying known and novel cell-type-specific DNA repair phenotypes.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Cell Line
DNA Repair*
Gene Expression*
Genomics
High-Throughput Nucleotide Sequencing
Humans
Lymphocytes / metabolism
Phenotype
RNA, Messenger / chemistry
RNA, Messenger / metabolism
RNA-Seq
Sequence Analysis, DNA
Single-Cell Analysis / methods*

Substances

RNA, Messenger

Abstract

Publication types

MeSH terms

Substances

Grants and funding