STRESS INDUCED FACTOR 2 Regulates Arabidopsis Stomatal Immunity through Phosphorylation of the Anion Channel SLAC1

Ching Chan; Dario Panzeri; Eiji Okuma; Kadri Tõldsepp; Ya-Yun Wang; Guan-Yu Louh; Tzu-Chuan Chin; Yu-Hung Yeh; Hung-Ling Yeh; Shweta Yekondi; You-Huei Huang; Tai-Yuan Huang; Tzyy-Jen Chiou; Yoshiyuki Murata; Hannes Kollist; Laurent Zimmerli

doi:10.1105/tpc.19.00578

STRESS INDUCED FACTOR 2 Regulates Arabidopsis Stomatal Immunity through Phosphorylation of the Anion Channel SLAC1

Plant Cell. 2020 Jul;32(7):2216-2236. doi: 10.1105/tpc.19.00578. Epub 2020 Apr 23.

Authors

Ching Chan^{1

2}, Dario Panzeri¹, Eiji Okuma³, Kadri Tõldsepp⁴, Ya-Yun Wang¹, Guan-Yu Louh¹, Tzu-Chuan Chin¹, Yu-Hung Yeh¹, Hung-Ling Yeh², Shweta Yekondi¹, You-Huei Huang¹, Tai-Yuan Huang¹, Tzyy-Jen Chiou², Yoshiyuki Murata³, Hannes Kollist⁴, Laurent Zimmerli⁵

Affiliations

¹ Department of Life Science and Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan.
² Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan.
³ Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.
⁴ University of Tartu, Tartu 50411, Estonia.
⁵ Department of Life Science and Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan [email protected].

Abstract

Upon recognition of microbes, pattern recognition receptors (PRRs) activate pattern-triggered immunity. FLAGELLIN SENSING2 (FLS2) and BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 (BAK1) form a typical PRR complex that senses bacteria. Here, we report that the kinase activity of the malectin-like receptor-like kinase STRESS INDUCED FACTOR 2 (SIF2) is critical for Arabidopsis (Arabidopsis thaliana) resistance to bacteria by regulating stomatal immunity. SIF2 physically associates with the FLS2-BAK1 PRR complex and interacts with and phosphorylates the guard cell SLOW ANION CHANNEL1 (SLAC1), which is necessary for abscisic acid (ABA)-mediated stomatal closure. SIF2 is also required for the activation of ABA-induced S-type anion currents in Arabidopsis protoplasts, and SIF2 is sufficient to activate SLAC1 anion channels in Xenopus oocytes. SIF2-mediated activation of SLAC1 depends on specific phosphorylation of Ser 65. This work reveals that SIF2 functions between the FLS2-BAK1 initial immunity receptor complex and the final actuator SLAC1 in stomatal immunity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abscisic Acid / metabolism
Abscisic Acid / pharmacology
Animals
Arabidopsis / microbiology
Arabidopsis / physiology*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / immunology
Arabidopsis Proteins / metabolism*
Disease Resistance / physiology
Female
Histone Deacetylases / genetics
Histone Deacetylases / immunology
Histone Deacetylases / metabolism*
Membrane Proteins / genetics
Membrane Proteins / immunology
Membrane Proteins / metabolism*
Mutation
Oocytes / physiology
Phosphorylation
Plant Diseases / immunology
Plant Diseases / microbiology
Plant Immunity / drug effects
Plant Stomata / immunology*
Plant Stomata / metabolism
Plants, Genetically Modified
Protein Kinases / metabolism
Protein Serine-Threonine Kinases / metabolism
Repressor Proteins / genetics
Repressor Proteins / immunology
Repressor Proteins / metabolism*
Serine / metabolism
Xenopus

Substances

Arabidopsis Proteins
Membrane Proteins
Repressor Proteins
SLAC1 protein, Arabidopsis
Serine
Abscisic Acid
Protein Kinases
BAK1 protein, Arabidopsis
FLS2 protein, Arabidopsis
OST1 protein, Arabidopsis
Protein Serine-Threonine Kinases
Histone Deacetylases
SIF2 protein, Arabidopsis