Naturally occurring variants in the transmembrane and cytoplasmic domains of the human Coxsackie- and adenovirus receptor have no impact on virus internalisation

Leonie Herrmann; Adrian Filip; Dennis Lapuente; Matthias Tenbusch; Karsten Niehaus; Volker Rudolph; Martin Farr

doi:10.1016/j.bbrc.2020.03.082

Naturally occurring variants in the transmembrane and cytoplasmic domains of the human Coxsackie- and adenovirus receptor have no impact on virus internalisation

Biochem Biophys Res Commun. 2020 Jun 25;527(2):401-405. doi: 10.1016/j.bbrc.2020.03.082. Epub 2020 Apr 23.

Authors

Leonie Herrmann¹, Adrian Filip², Dennis Lapuente³, Matthias Tenbusch⁴, Karsten Niehaus⁵, Volker Rudolph⁶, Martin Farr⁷

Affiliations

¹ Clinic for General and Interventional Cardiology/Angiology, Herz- und Diabeteszentrum NRW, Ruhr-Universität Bochum, 32545, Bad Oeynhausen, Germany; Proteom- und Metabolomforschung, Fakultät für Biologie & CeBiTec, Universität Bielefeld, 33501, Bielefeld, Germany. Electronic address: [email protected].
² Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Schlossgarten 4, 91054, Erlangen, Germany. Electronic address: [email protected].
³ Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Schlossgarten 4, 91054, Erlangen, Germany. Electronic address: [email protected].
⁴ Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Schlossgarten 4, 91054, Erlangen, Germany. Electronic address: [email protected].
⁵ Proteom- und Metabolomforschung, Fakultät für Biologie & CeBiTec, Universität Bielefeld, 33501, Bielefeld, Germany. Electronic address: [email protected].
⁶ Clinic for General and Interventional Cardiology/Angiology, Herz- und Diabeteszentrum NRW, Ruhr-Universität Bochum, 32545, Bad Oeynhausen, Germany. Electronic address: [email protected].
⁷ Clinic for General and Interventional Cardiology/Angiology, Herz- und Diabeteszentrum NRW, Ruhr-Universität Bochum, 32545, Bad Oeynhausen, Germany. Electronic address: [email protected].

PMID: 32334832
DOI: 10.1016/j.bbrc.2020.03.082

Abstract

The Coxsackie- and adenovirus receptor (CAR) mediates homophilic cell-cell contacts and susceptibility to both human pathogenic viruses through its membrane-distal immunoglobulin domain. In the present study, we screened five missense variants of the human CAR gene for their influence on adenovector or Coxsackievirus entry into Chinese hamster ovary cells. The CAR variants facilitated virus internalisation to a similar extent as wild type CAR. This underlines CAR's presumed invariance and essential physiological role in embryogenesis.

Keywords: Coxsackievirus and adenovirus receptor; Genetic variants; Viral infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CHO Cells
Coxsackie and Adenovirus Receptor-Like Membrane Protein / chemistry
Coxsackie and Adenovirus Receptor-Like Membrane Protein / genetics*
Coxsackie and Adenovirus Receptor-Like Membrane Protein / metabolism
Coxsackievirus Infections / genetics*
Coxsackievirus Infections / metabolism
Cricetulus
Enterovirus / physiology*
Host-Pathogen Interactions
Humans
Mutation, Missense*
Protein Domains
Virus Internalization*

Substances

Coxsackie and Adenovirus Receptor-Like Membrane Protein