NDRG1-PLAG1 and TRPS1-PLAG1 Fusion Genes in Chondroid Syringoma

Cancer Genomics Proteomics. 2020 May-Jun;17(3):237-248. doi: 10.21873/cgp.20184.

Abstract

Background/aim: Chondroid syringoma is a rare benign tumor emanating from sweat glands. Although rearrangements of the pleomorphic adenoma gene 1 (PLAG1) have been reported in such tumors, information on PLAG1 fusion genes is very limited.

Materials and methods: Cytogenetic, fluorescence in situ hybridization, RNA sequencing, array comparative genomic hybridization, reverse transcription polymerase chain reaction, and Sanger sequencing analyses were performed on two chondroid syringoma cases.

Results: Both tumors had structural rearrangements of chromosome 8. An NDRG1-PLAG1 transcript was found in the first tumor in which exon 3 of PLAG1 was fused with exon 1 of NDRG1. A TRPS1-PLAG1 chimeric transcript was detected in the second chondroid syringoma in which exon 2 or exon 3 of PLAG1 was fused with exon 1 of TRPS1.

Conclusion: The NDRG1-PLAG1 and TRPS1-PLAG1 resemble other PLAG1 fusion genes inasmuch as the expression of PLAG1 comes under the control of the NDRG1 or TRPS1 promoter.

Keywords: Chondroid syringoma; NDRG1-PLAG1; PLAG1; TRPS1-PLAG1; chromosome 8; fusion genes.

Publication types

  • Case Reports

MeSH terms

  • Adenoma, Pleomorphic / genetics*
  • Adenoma, Pleomorphic / pathology
  • Biomarkers, Tumor / genetics
  • Cell Cycle Proteins / genetics*
  • Comparative Genomic Hybridization / methods
  • DNA-Binding Proteins / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence / methods
  • Intracellular Signaling Peptides and Proteins / genetics*
  • Male
  • Middle Aged
  • Oncogene Proteins, Fusion / genetics*
  • Repressor Proteins / genetics*
  • Sequence Analysis, RNA / methods
  • Sweat Gland Neoplasms / genetics*
  • Sweat Gland Neoplasms / pathology

Substances

  • Biomarkers, Tumor
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Intracellular Signaling Peptides and Proteins
  • N-myc downstream-regulated gene 1 protein
  • Oncogene Proteins, Fusion
  • PLAG1 protein, human
  • Repressor Proteins
  • TRPS1 protein, human