Generation of induced pluripotent stem cell line (XDCMHi001-A) from an Ankylosing spondylitis patient with JAK2 mutation

Jintao Hu; Weifan Ren; Weiguo Qiu; Jianlan Lv; Cui Zhang; Chao Xu; Weibin DU; Mengrui Wu; Jinfu Wang; Renfu Quan

doi:10.1016/j.scr.2020.101788

Generation of induced pluripotent stem cell line (XDCMHi001-A) from an Ankylosing spondylitis patient with JAK2 mutation

Stem Cell Res. 2020 May:45:101788. doi: 10.1016/j.scr.2020.101788. Epub 2020 Apr 20.

Authors

Jintao Hu¹, Weifan Ren², Weiguo Qiu², Jianlan Lv², Cui Zhang³, Chao Xu⁴, Weibin DU⁵, Mengrui Wu³, Jinfu Wang⁶, Renfu Quan⁷

Affiliations

¹ The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China; Department of Orthopedics, Hangzhou Xiaoshan District Chinese Medicine Hospital, Hangzhou 311200, China.
² The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
³ Laboratory of Stem Cells, Institute of Cell Biology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
⁴ Department of Orthopedics, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310000, China.
⁵ Department of Orthopedics, Hangzhou Xiaoshan District Chinese Medicine Hospital, Hangzhou 311200, China.
⁶ Laboratory of Stem Cells, Institute of Cell Biology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China. Electronic address: [email protected].
⁷ The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China; Department of Orthopedics, Hangzhou Xiaoshan District Chinese Medicine Hospital, Hangzhou 311200, China. Electronic address: [email protected].

PMID: 32388440
DOI: 10.1016/j.scr.2020.101788

Abstract

Heredity is the major factor contributing to the susceptibility to ankylosing spondylitis(AS). Janus kinase 2 (JAK2) has been associated with AS. Urine-derived cells from an AS patient with JAK2 mutation were used to generate induced pluripotent stem cells (iPSCs) with five episomal iPSC reprogramming vectors (pCXLE-hOCT3/4-shp53-F, pCXLE-hSK, pCXLE-hUL, pCXLE-EGFP and pCXWB-EBNA1). The iPSCs were pluripotent and will be valuable for research on the role and mechanism of JAK2 in the pathogenesis of AS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cellular Reprogramming
Humans
Induced Pluripotent Stem Cells*
Janus Kinase 2 / genetics
Mutation
Plasmids
Spondylitis, Ankylosing* / genetics

Substances

JAK2 protein, human
Janus Kinase 2