HDAC1 modulates OGG1-initiated oxidative DNA damage repair in the aging brain and Alzheimer's disease

Nat Commun. 2020 May 18;11(1):2484. doi: 10.1038/s41467-020-16361-y.

Abstract

DNA damage contributes to brain aging and neurodegenerative diseases. However, the factors stimulating DNA repair to stave off functional decline remain obscure. We show that HDAC1 modulates OGG1-initated 8-oxoguanine (8-oxoG) repair in the brain. HDAC1-deficient mice display age-associated DNA damage accumulation and cognitive impairment. HDAC1 stimulates OGG1, a DNA glycosylase known to remove 8-oxoG lesions that are associated with transcriptional repression. HDAC1 deficiency causes impaired OGG1 activity, 8-oxoG accumulation at the promoters of genes critical for brain function, and transcriptional repression. Moreover, we observe elevated 8-oxoG along with reduced HDAC1 activity and downregulation of a similar gene set in the 5XFAD mouse model of Alzheimer's disease. Notably, pharmacological activation of HDAC1 alleviates the deleterious effects of 8-oxoG in aged wild-type and 5XFAD mice. Our work uncovers important roles for HDAC1 in 8-oxoG repair and highlights the therapeutic potential of HDAC1 activation to counter functional decline in brain aging and neurodegeneration.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Aging / genetics
  • Aging / pathology*
  • Alzheimer Disease / complications
  • Alzheimer Disease / pathology*
  • Alzheimer Disease / physiopathology
  • Animals
  • Astrocytes / drug effects
  • Astrocytes / metabolism
  • Astrocytes / pathology
  • Base Sequence
  • Benzophenones / pharmacology
  • Brain / pathology*
  • Cognition / drug effects
  • Cognition Disorders / complications
  • Cognition Disorders / pathology
  • DNA Damage*
  • DNA Glycosylases / metabolism*
  • Down-Regulation / drug effects
  • Down-Regulation / genetics
  • Gene Ontology
  • Guanine / analogs & derivatives
  • Guanine / metabolism
  • Histone Deacetylase 1 / metabolism*
  • Memory / drug effects
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Neurons / drug effects
  • Neurons / metabolism
  • Oxidative Stress* / drug effects
  • Promoter Regions, Genetic / genetics

Substances

  • Benzophenones
  • exifone
  • 8-hydroxyguanine
  • Guanine
  • DNA Glycosylases
  • Ogg1 protein, mouse
  • Hdac1 protein, mouse
  • Histone Deacetylase 1