Effect of hnRNPA2/B1 on the proliferation and apoptosis of glioma U251 cells via the regulation of AKT and STAT3 pathways

Biosci Rep. 2020 Jul 31;40(7):BSR20190318. doi: 10.1042/BSR20190318.

Abstract

Glioma is the most common malignant tumor in the human central nervous system. Although heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2/B1) was previously presumed to be a tumor-promoting gene, the relationship between hnRNPA2/B1 and glioma is unclear. Targeting hnRNPA2/B1 interference in glioma cells can significantly inhibit proliferation and increase apoptosis of human glioma cells in vitro. In a tumor xenograft model, knockdown of hnRNPA2/B1 suppressed tumor growth in glioma cells in vivo. In terms of a mechanism, the knockdown of hnRNPA2/B1 led to inactivation of the AKT and STAT3 signaling pathways, which ultimately reduced the expression of B-cell lymphoma-2 (Bcl-2), CyclinD1 and proliferating cell nuclear antigen (PCNA). Collectively, these data suggest that the inhibition of hnRNPA2/B1 can reduce the growth of gliomas through STAT3 and AKT signaling pathways, and this inhibition is expected to be a therapeutic target for gliomas.

Keywords: apoptosis; glioma; hnRNPA2/B1; proliferation.

MeSH terms

  • Alternative Splicing
  • Animals
  • Apoptosis / genetics
  • Brain Neoplasms / genetics*
  • Brain Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Proliferation / genetics
  • Gene Expression Regulation, Neoplastic*
  • Gene Knockdown Techniques
  • Glioma / genetics*
  • Glioma / pathology
  • Heterogeneous-Nuclear Ribonucleoprotein Group A-B / genetics
  • Heterogeneous-Nuclear Ribonucleoprotein Group A-B / metabolism*
  • Humans
  • Male
  • Mice
  • Proto-Oncogene Proteins c-akt / metabolism
  • RNA Interference
  • STAT3 Transcription Factor / metabolism
  • Signal Transduction / genetics
  • Xenograft Model Antitumor Assays

Substances

  • Heterogeneous-Nuclear Ribonucleoprotein Group A-B
  • STAT3 Transcription Factor
  • STAT3 protein, human
  • hnRNP A2
  • AKT1 protein, human
  • Proto-Oncogene Proteins c-akt