16S rRNA Amplicon Sequencing of Urban Prokaryotic Communities in the South Bronx River Estuary

Eugenia Naro-Maciel; Melissa R Ingala; Irena E Werner; Allison M Fitzgerald

doi:10.1128/MRA.00182-20

16S rRNA Amplicon Sequencing of Urban Prokaryotic Communities in the South Bronx River Estuary

Microbiol Resour Announc. 2020 May 28;9(22):e00182-20. doi: 10.1128/MRA.00182-20.

Authors

Eugenia Naro-Maciel¹, Melissa R Ingala², Irena E Werner³, Allison M Fitzgerald⁴

Affiliations

¹ Liberal Studies, New York University, New York, New York, USA [email protected].
² Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, New York, USA.
³ Biology Department, College of Staten Island, City University of New York, Staten Island, New York, USA.
⁴ New Jersey City University, Jersey City, New Jersey, USA.

Abstract

Biodiversity monitoring is an essential component of restoration efforts. We sequenced 16S rRNA gene amplicons from sediments and waters of Hunts Point Riverside Park and Soundview Park, located in a historically degraded but recovering urban estuary in New York. In total, 16,165 unique amplicon sequence variants were recovered, and Proteobacteria was the dominant phylum.