Biochemical, electrophysiological and immunological characterization of the venom from Centruroides baergi, a new scorpion species of medical importance in Mexico

Toxicon. 2020 Sep:184:10-18. doi: 10.1016/j.toxicon.2020.05.021. Epub 2020 May 30.

Abstract

In this communication the isolation, chemical and physiological characterization of three new toxins from the scorpion Centruroides baergi are reported. Their immunoreactive properties with scFvs generated in our group are described. The three new peptides, named Cb1, Cb2 and Cb3 affect voltage-dependent Na+ channels in a differential manner. These characteristics, explain the toxicity of this venom. Molecular interactions in real-time among these toxins and the best recombinant antibodies generated in our group, revealed that one of them was able to neutralize the main toxin of this venom (Cb1). These results represent an important advance for the neutralization of this venom and serve as the basis for generating new scFvs that will allow the neutralization of similar toxins from other venoms that have no yet been neutralized.

Keywords: Centruroides baergi; Human scFvs; Scorpion β-toxins; Sodium channels; Venom neutralization.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Electrophysiological Phenomena
  • Mexico
  • Recombinant Proteins
  • Scorpion Venoms / analysis*
  • Scorpion Venoms / immunology
  • Scorpions*
  • Sequence Alignment
  • Single-Chain Antibodies

Substances

  • Recombinant Proteins
  • Scorpion Venoms
  • Single-Chain Antibodies