Assessment of Periprostatic and Subcutaneous Adipose Tissue Lipolysis and Adipocyte Size from Men with Localized Prostate Cancer

Dushan Miladinovic; Thomas Cusick; Kate L Mahon; Anne-Maree Haynes; Colin H Cortie; Barbara J Meyer; Phillip D Stricker; Gary A Wittert; Lisa M Butler; Lisa G Horvath; Andrew J Hoy

doi:10.3390/cancers12061385

Assessment of Periprostatic and Subcutaneous Adipose Tissue Lipolysis and Adipocyte Size from Men with Localized Prostate Cancer

Cancers (Basel). 2020 May 28;12(6):1385. doi: 10.3390/cancers12061385.

Authors

Dushan Miladinovic¹, Thomas Cusick², Kate L Mahon^{2

3

4

5}, Anne-Maree Haynes², Colin H Cortie^{6

7}, Barbara J Meyer^{6

7}, Phillip D Stricker^{2

8

9}, Gary A Wittert^{10

11}, Lisa M Butler^{10

11}, Lisa G Horvath^{2

3

4

5}, Andrew J Hoy¹

Affiliations

¹ Discipline of Physiology, School of Medical Sciences, Charles Perkins Centre, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia.
² Cancer Division, The Kinghorn Cancer Centre/Garvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia.
³ Discipline of Medicine, Central Clinical School, The University of Sydney School of Medicine, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia.
⁴ Department of Medical Oncology, Chris O'Brien Lifehouse, Camperdown, NSW 2050, Aistralia.
⁵ Royal Prince Alfred Hospital, Camperdown, NSW 2050, Australia.
⁶ School of Medicine, Lipid Research Centre, Molecular Horizons, University of Wollongong, Wollongong, NSW 2522, Australia.
⁷ Illawarra Medical Research Institute, University of Wollongong, Wollongong NSW 2522, Australia.
⁸ St. Vincent's Clinical School, The University of New South Wales, Sydney, NSW 2052, Australia.
⁹ St. Vincent's Prostate Cancer Centre, St. Vincent's Clinic, Darlinghurst, NSW 2010, Australia.
¹⁰ South Australian Health and Medical Research Institute, Adelaide, SA 5000, Australia.
¹¹ School of Medicine and Freemasons Foundation Centre for Men's Health, University of Adelaide, Adelaide, SA 5005, Australia.

Abstract

The prostate is surrounded by periprostatic adipose tissue (PPAT), the thickness of which has been associated with more aggressive prostate cancer (PCa). There are limited data regarding the functional characteristics of PPAT, how it compares to subcutaneous adipose tissue (SAT), and whether in a setting of localized PCa, these traits are altered by obesity or disease aggressiveness. PPAT and SAT were collected from 60 men (age: 42-78 years, BMI: 21.3-35.6 kg/m²) undergoing total prostatectomy for PCa. Compared to SAT, adipocytes in PPAT were smaller, had the same basal rates of fatty acid release (lipolysis) yet released less polyunsaturated fatty acid species, and were more sensitive to isoproterenol-stimulated lipolysis. Basal lipolysis of PPAT was increased in men diagnosed with less aggressive PCa (Gleason score (GS) ≤ 3 + 4) compared to men with more aggressive PCa (GS ≥ 4 + 3) but no other measured adipocyte parameters related to PCa aggressiveness. Likewise, there was no difference in PPAT lipid biology between lean and obese men. In conclusion, lipid biological features of PPAT do differ from SAT; however, we did not observe any meaningful difference in ex vivo PPAT biology that is associated with PCa aggressiveness or obesity. As such, our findings do not support a relationship between altered PCa behavior in obese men and the metabolic reprogramming of PPAT.

Keywords: lipolysis; localized prostate cancer; periprostatic adipose tissue; prostate cancer; subcutaneous adipose tissue.

Abstract

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