Improvement of selection of pregnant women for intrapartum polymerase chain reaction screening for vaginal Group B Streptococci (GBS) colonization by adding GBS urine screening at 35-37 weeks of pregnancy

Int J Gynaecol Obstet. 2020 Oct;151(1):124-127. doi: 10.1002/ijgo.13267. Epub 2020 Jul 9.

Abstract

Objective: To evaluate whether systematic antepartum screening for Group B Streptococci (GBS) by urine culture improves the risk factor-based selection of pregnant women for intrapartum GBS screening with a rapid polymerase chain reaction (PCR) assay.

Methods: A prospective observational study was conducted between April 2013 and June 2014. GBS colonization judged by urine culture at 35-37 weeks of gestation was compared with the result of a vaginal GBS PCR test at labor as outcome. The results of urine culture were used as exposure variable. The PCR test was performed on intrapartum vaginal samples.

Results: Screening for urine GBS in 902 unselected pregnant Danish women at 35-37 weeks of gestation predicted intrapartum PCR GBS status with a sensitivity of 33.6%. A positive predictive value of 41.2% was seen among women with low GBS counts (<104 CFU/mL) and 83.3% among women with high GBS counts (≥104 CFU/mL). Systematic GBS screening of urine at 35-37 weeks of gestation added 30.9% extra women for intrapartum GBS PCR screening in the study group.

Conclusion: Systematic antepartum GBS screening of urine should be implemented in order to improve risk stratification for early onset GBS by offering laboring women an intrapartum GBS PCR test.

Keywords: Asymptomatic bacteriuria; Early onset of neonatal GBS disease; Group B streptococci; Polymerase chain reaction.

Publication types

  • Observational Study

MeSH terms

  • Adult
  • Female
  • Humans
  • Patient Selection*
  • Predictive Value of Tests
  • Pregnancy
  • Pregnancy Complications, Infectious / diagnosis*
  • Prospective Studies
  • Real-Time Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Streptococcal Infections / diagnosis*
  • Streptococcal Infections / urine
  • Streptococcus agalactiae / genetics*
  • Streptococcus agalactiae / isolation & purification
  • Urinalysis*