COVID-19: molecular diagnostics overview

J Mol Med (Berl). 2020 Jul;98(7):947-954. doi: 10.1007/s00109-020-01931-w. Epub 2020 Jun 13.

Abstract

The last day of 2019 delivered the first report to the World Health Organization (WHO) about a group of cases of pneumonia of unknown etiology in Wuhan, China. Subsequent investigations identified the new comer, a novel coronavirus related to severe acute respiratory syndrome coronavirus (SARS-CoV) and thus was termed as SARS-CoV-2. Being very contagious, the new virus led the era of "COVID-19" which is the acronym of "coronavirus disease 2019," evoking an imminent threat to global health security with unprecedented devastating challenges to human kind. In this article, we provide a molecular overview on the SARS-CoV-2 virus and summarize tremendous efforts that have been made to develop a rapid confirmatory diagnostic test for COVID-19. The diagnostic performances of the available tests are analyzed based on the best current information from the early research.

Keywords: COVID-19; Coronavirus; SARS-CoV-2.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Antibodies, Viral / blood*
  • Betacoronavirus / genetics*
  • Betacoronavirus / immunology*
  • COVID-19
  • China
  • Coronavirus Infections / diagnosis*
  • Coronavirus Infections / physiopathology
  • Coronavirus Nucleocapsid Proteins
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Nucleocapsid Proteins / immunology
  • Pandemics
  • Phosphoproteins
  • Pneumonia, Viral / diagnosis*
  • Pneumonia, Viral / physiopathology
  • RNA, Viral / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • SARS-CoV-2
  • Spike Glycoprotein, Coronavirus / immunology

Substances

  • Antibodies, Viral
  • Coronavirus Nucleocapsid Proteins
  • Nucleocapsid Proteins
  • Phosphoproteins
  • RNA, Viral
  • Spike Glycoprotein, Coronavirus
  • nucleocapsid phosphoprotein, SARS-CoV-2
  • spike protein, SARS-CoV-2