The curious fate of bone following bariatric surgery: bone effects of sleeve gastrectomy (SG) and Roux-en-Y gastric bypass (RYGB) in mice

Int J Obes (Lond). 2020 Oct;44(10):2165-2176. doi: 10.1038/s41366-020-0626-3. Epub 2020 Jun 16.

Abstract

Background: Bone loss and increased fracture risk following bariatric surgery has been reported. We investigated whether the two most commonly performed surgeries, sleeve gastrectomy (SG) and Roux-en-Y gastric bypass (RYGB), lead to bone loss. In addition, we examined whether fortification of the diet with calcium citrate prevents bone loss.

Methods: We used mouse models for SG and RYGB and compared bone loss with a group of sham mice with similar weight loss. All groups were switched at the time of surgery to a low-fat diet (LFD). We also examined whether fortification of the diet with calcium citrate and vitamin D was able to prevent bone loss.

Results: At 2 weeks we observed no major bone effects. However, at 8 weeks, both trabecular and cortical bone were lost to the same extent after SG and RYGB, despite increased calcium absorption and adequate serum levels of calcium, vitamin D, and parathyroid hormone (PTH). Diet fortification with calcium citrate and vitamin D was able to partially prevent bone loss.

Conclusions: Both SG and RYGB lead to excess bone loss, despite intestinal adaptations to increase calcium absorption. Fortifying the diet with calcium citrate and vitamin D partly prevented the observed bone loss. This finding emphasizes the importance of nutritional support strategies after bariatric surgery, but also affirms that the exact mechanisms leading to bone loss after bariatric surgery remain elusive and thus warrant further research.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Resorption / etiology*
  • Bone Resorption / prevention & control
  • Calcium / administration & dosage
  • Calcium / blood
  • Diet
  • Dietary Supplements
  • Eating
  • Gastrectomy / adverse effects*
  • Gastric Bypass / adverse effects*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Parathyroid Hormone / blood
  • Vitamin D / administration & dosage
  • Vitamin D / blood
  • Weight Loss

Substances

  • Parathyroid Hormone
  • Vitamin D
  • Calcium