Liposomal Encapsulated Curcumin Effectively Attenuates Neuroinflammatory and Reactive Astrogliosis Reactions in Glia Cells and Organotypic Brain Slices

Int J Nanomedicine. 2020 May 25:15:3649-3667. doi: 10.2147/IJN.S245300. eCollection 2020.

Abstract

Introduction: The polyphenolic spice and food coloring ingredient curcumin has beneficial effects in a broad variety of inflammatory diseases. Amongst them, curcumin has been shown to attenuate microglia reaction and prevent from glial scar formation in spinal cord and brain injuries.

Methods: We developed a protocol for the efficient encapsulation of curcumin as a model for anti-inflammatory drugs yielding long-term stable, non-toxic liposomes with favorable physicochemical properties. Subsequently, we evaluate the effects of liposomal curcumin in experimental models for neuroinflammation and reactive astrogliosis.

Results: We could show that liposomal curcumin can efficiently reduce the reactivity of human microglia and astrocytes and preserve tissue integrity of murine organotypic cortex slices.

Discussion and perspective: In perspective, we want to administer this curcumin formulation in brain implant coatings to prevent neuroinflammation and glial scar formation as foreign body responses of the brain towards implanted materials.

Keywords: brain implants; glial scar; nanoparticles; neuroinflammation.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Astrocytes / drug effects
  • Astrocytes / ultrastructure
  • Brain / drug effects
  • Brain / pathology*
  • Cell Line
  • Cell Survival / drug effects
  • Curcumin / pharmacology
  • Curcumin / therapeutic use*
  • Gliosis / drug therapy*
  • Humans
  • Inflammation / drug therapy*
  • Inflammation / pathology
  • Lipopolysaccharides / pharmacology
  • Liposomes
  • Mice
  • Microglia / drug effects
  • Microglia / ultrastructure
  • Neuroglia / drug effects
  • Neuroglia / pathology*

Substances

  • Anti-Inflammatory Agents
  • Lipopolysaccharides
  • Liposomes
  • Curcumin