High-performance thin-layer chromatography fingerprinting, total phenolic and total flavonoid contents and anti-platelet-aggregation activities of Prosopis farcta extracts

Cell Mol Biol (Noisy-le-grand). 2020 Jun 25;66(4):8-14.

Abstract

Cardiovascular diseases are a leading cause of worldwide death and excessive platelet is closely related with their pathogenesis. Different plants and natural compounds have demonstrated anti-platelet effects. The aim of this study was to report the high-performance thin-layer chromatography (HPTLC) fingerprinting and anti-platelet-aggregation activities of different leaf extracts (n-hexane, chloroform, ethyl acetate, methanol and aqueous) of Prosopis farcta (Syrian mesquite) plant. The results showed a 100% inhibition of aggregation activity after plasmatic adenosine diphosphate (ADP) aggregation activation of ethyl acetate, ethanolic, methanolic and aqueous extracts, at 60 mg/mL concentration. The IC50 ADP value of these extracts ranged between 4.07 and 11.39 mg/mL. Moreover, these extracts reported the highest amounts of phenolic and flavonoid contents. In conclusion, phytochemicals present in P. farcta leaves have anti-platelet-aggregation activities. Future studies are needed to identify the compounds with anti-platelet potential present in P. farcta.

Keywords: Antiplatelet; Cardiovascular disease; HPTLC.; Phytochemical; Syrian mesquite.

MeSH terms

  • Antioxidants / analysis
  • Antioxidants / pharmacology
  • Chromatography, Thin Layer / methods*
  • Flavonoids / analysis*
  • Humans
  • Phenols / analysis*
  • Plant Extracts / pharmacology*
  • Platelet Aggregation / drug effects*
  • Prosopis / chemistry*
  • Reference Standards
  • Terpenes / analysis
  • Terpenes / pharmacology

Substances

  • Antioxidants
  • Flavonoids
  • Phenols
  • Plant Extracts
  • Terpenes