Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues

Seizo Fujikawa; Keita Endoh

doi:10.1007/978-1-0716-0660-5_9

Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues

Methods Mol Biol. 2020:2156:99-117. doi: 10.1007/978-1-0716-0660-5_9.

Authors

Seizo Fujikawa¹, Keita Endoh²

Affiliations

¹ Faculty of Agriculture, Hokkaido University, Sapporo, Japan. [email protected].
² Forestry and Forest Products Research Institute, Forest Tree Breeding Center, Hitachi, Japan.

PMID: 32607978
DOI: 10.1007/978-1-0716-0660-5_9

Abstract

A cryo-scanning electron microscope (cryo-SEM) is a valuable tool for observing bulk frozen samples to monitor freezing responses of plant tissues and cells. Here, the essential processes of a cryo-SEM to observe freezing behaviors of plant tissue cells are described.

Keywords: Cooling rate; Cryo-fixation; Cryo-scanning electron microscope (cryo-SEM); Extracellular freezing; Ice crystal; Intracellular freezing; Recrystallization.

MeSH terms

Acclimatization*
Cell Wall / ultrastructure
Cryoelectron Microscopy*
Freezing*
Microscopy, Electron, Scanning*
Plant Physiological Phenomena*