Abstract
Sib selection and an ELISA have been used to isolate hybridoma subclones producing mutant antibodies that bind antigen better than the parental monoclonal antibody. Such mutants arise spontaneously in culture at frequencies of 2.5-5 X 10(-5). The sequences of the heavy and light chain variable regions of the mutant antibodies are identical to that of the parent and the Ka values of the mutants and the parent are the same. The increase in binding is associated with abnormalities of the constant region polypeptide and probably reflect changes in avidity of these antibodies.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal / genetics
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Antibodies, Monoclonal / immunology*
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Antibodies, Monoclonal / metabolism
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Antigen-Antibody Reactions*
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Antigens / metabolism
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Base Sequence
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Enzyme-Linked Immunosorbent Assay
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Hybridomas / immunology
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Immunoglobulin G / immunology
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Immunoglobulin Variable Region / genetics
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Mice
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Protein Binding
Substances
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Antibodies, Monoclonal
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Antigens
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Immunoglobulin G
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Immunoglobulin Variable Region
Associated data
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GENBANK/M20274
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GENBANK/M20275
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GENBANK/M20276
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GENBANK/M20277
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GENBANK/M20278
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GENBANK/M20279
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GENBANK/M20280
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GENBANK/M20281