Targeted molecular diagnostic tests and accurate immunoassays have transformed the landscape of clinical virology, calling into question the usefulness of traditional viral culture. Here we present a case where viral culture, followed by metagenomic sequencing, was central to the diagnosis of an unexpected viral infection, with significant clinical and public health implications.
Keywords: metagenomic sequencing; poxvirus; vaccine-transmitted; vaccinia; viral culture.
© The Author(s) 2020. Published by Oxford University Press on behalf of Infectious Diseases Society of America.