Genome editing of Francisella tularensis using (CRISPR-Cas9)

Stephanie J Southern; Petra C F Oyston

doi:10.1016/j.mimet.2020.106004

Genome editing of Francisella tularensis using (CRISPR-Cas9)

J Microbiol Methods. 2020 Sep:176:106004. doi: 10.1016/j.mimet.2020.106004. Epub 2020 Jul 18.

Authors

Stephanie J Southern¹, Petra C F Oyston²

Affiliations

¹ Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK.
² Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK. Electronic address: [email protected].

PMID: 32687866
DOI: 10.1016/j.mimet.2020.106004

Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system is a powerful tool for gene editing in eukaryotic genomes but is still being developed for editing bacterial genomes. Here we describe the construction of an all-in-one vector for generating potentially scarless deletion mutants in Francisella tularensis LVS using a CRISPR-Cas9-based system.

Keywords: CRISPR-Cas9; Francisella; Gene editing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems*
Francisella tularensis / genetics*
Gene Editing / methods*
Genome, Bacterial*