Abstract
In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
MeSH terms
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Animals
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Antibodies, Monoclonal
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Antibodies, Viral
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Antisense Elements (Genetics) / genetics
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Betacoronavirus / genetics*
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Betacoronavirus / isolation & purification*
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COVID-19
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COVID-19 Testing
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Chlorocebus aethiops
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Clinical Laboratory Techniques / methods*
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Coronavirus Infections / diagnosis*
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Coronavirus Infections / virology
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Coronavirus Nucleocapsid Proteins
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Genes, Viral
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Humans
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Immunohistochemistry / methods
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In Situ Hybridization / methods
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Nucleocapsid Proteins / genetics
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Nucleocapsid Proteins / metabolism
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Pandemics
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Phosphoproteins
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Pneumonia, Viral / diagnosis*
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Pneumonia, Viral / virology
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Polyproteins
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RNA, Viral / genetics*
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RNA, Viral / metabolism
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SARS-CoV-2
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Spike Glycoprotein, Coronavirus / genetics
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Spike Glycoprotein, Coronavirus / metabolism
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Vero Cells
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Viral Proteins / genetics
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Viral Proteins / metabolism
Substances
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Antibodies, Monoclonal
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Antibodies, Viral
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Antisense Elements (Genetics)
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Coronavirus Nucleocapsid Proteins
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Nucleocapsid Proteins
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ORF1ab polyprotein, SARS-CoV-2
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Phosphoproteins
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Polyproteins
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RNA, Viral
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Spike Glycoprotein, Coronavirus
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Viral Proteins
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nucleocapsid phosphoprotein, SARS-CoV-2
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spike protein, SARS-CoV-2