The root iron transporter 1 governs cadmium uptake in Vicia sativa roots

Xingxing Zhang; Xin Li; Ling Tang; Yizhe Peng; Meng Qian; Yafang Guo; Haiyun Rui; Fenqin Zhang; Zhubing Hu; Yahua Chen; Yan Xia; Zhenguo Shen

doi:10.1016/j.jhazmat.2020.122873

The root iron transporter 1 governs cadmium uptake in Vicia sativa roots

J Hazard Mater. 2020 Nov 5:398:122873. doi: 10.1016/j.jhazmat.2020.122873. Epub 2020 May 14.

Authors

Xingxing Zhang¹, Xin Li², Ling Tang², Yizhe Peng³, Meng Qian², Yafang Guo², Haiyun Rui⁴, Fenqin Zhang⁵, Zhubing Hu⁶, Yahua Chen⁷, Yan Xia⁸, Zhenguo Shen⁹

Affiliations

¹ College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China; Guangdong Engineering Research Center for Pesticide & Fertilizer, Guangdong Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute), Guangzhou, 510316, China.
² College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
³ College of Resources & Environment, Hunan Agricultural University, Changsha, 410128, China.
⁴ Taizhou University, Taizhou, 225300, China.
⁵ College of Agriculture and Biotechnology, Hexi University, Zhangye, 734000, China.
⁶ Center for Multi-Omics Research, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, 475004, China.
⁷ College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, Nanjing, 210095, China.
⁸ College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, Nanjing, 210095, China. Electronic address: [email protected].
⁹ College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, Nanjing, 210095, China. Electronic address: [email protected].

PMID: 32768815
DOI: 10.1016/j.jhazmat.2020.122873

Abstract

Cadmium is a non-essential element for plants and that inhibits plant growth and development. The Zhangye Mawan (ZM) variety of Vicia sativa is more sensitive to Cd toxicity than that Lanjian 3# (L3) variety, but the underlying mechanism is not fully understood. Here, we demonstrated that ZM showed higher Cd accumulation than L3 based on root Cd content and Cd fluorescence intensity in root protoplasts. VsRIT1, a member of the ZIP (ZRT/IRT-like protein) family, showed expression levels in ZM roots 8-fold higher than those in L3 roots under Cd exposure. VsRIT1 expression increased Cd transport and accumulation in Arabidopsis and yeast. These suggests that VsRIT1 participates in Cd uptake by V. sativa roots. Furthermore, ZM root tips have a higher capacity for transient Cd influx than L3 roots when exposed to Cd alone or Cd and iron (Fe) together, owing to the higher VsRIT1 expression in ZM. Our findings also imply that Cd may compete with Fe or/and zinc (Zn) for uptake via VsRIT1 in V. sativa or yeast.

Keywords: Competitive uptake; Root iron transporter 1,Cd toxicity; Transient Cd influx; Vicia sativa.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cadmium* / toxicity
Iron
Plant Roots
Vicia sativa*
Zinc

Substances

Cadmium
Iron
Zinc