Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

Biomed Res Int. 2020 Jul 24:2020:7035847. doi: 10.1155/2020/7035847. eCollection 2020.

Abstract

Gliomas are the most common primary tumors in the brain with poor prognosis. Previous studies have detected high expression of Cyclophilin A (CyPA) and CD147, respectively, in glioma. However, the correlation between their expressions and glioma prognosis remains unclear. Here, we investigated the expression of CyPA and CD147 in different types of glioma and characterized their relationships with clinical features, prognosis, and cell proliferation. Results showed that CyPA and CD147 expressions were elevated in higher grade gliomas. Moreover, the knockdown of CyPA and CD147 by RNA interference significantly induced cell express apoptosis biomarkers such as Annexin V and inhibited proliferation biomarkers like EdU in glioma cells. In summary, our findings revealed that high expression of CyPA and CD147 correlated with glioma grades. Moreover, downregulation of the Cyclophilin A/CD147 axis induces cell apoptosis and inhibits glioma aggressiveness. Those indicating CyPA and CD147 could be used as both potential predictive biomarkers and a potential therapeutic target.

MeSH terms

  • Annexin A5 / biosynthesis
  • Annexin A5 / genetics
  • Apoptosis
  • Basigin / biosynthesis*
  • Basigin / genetics
  • Brain Neoplasms / genetics
  • Brain Neoplasms / metabolism*
  • Brain Neoplasms / mortality
  • Brain Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Proliferation*
  • Cyclophilin A / biosynthesis*
  • Cyclophilin A / genetics
  • Down-Regulation*
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Glioma / genetics
  • Glioma / metabolism*
  • Glioma / mortality
  • Humans
  • Male
  • Neoplasm Proteins / biosynthesis*
  • Neoplasm Proteins / genetics

Substances

  • Annexin A5
  • BSG protein, human
  • Neoplasm Proteins
  • Basigin
  • Cyclophilin A