Gold nanoparticle based immunochromatographic biosensor for rapid diagnosis of Mycobacterium avium subspecies paratuberculosis infection using recombinant protein

Aditya Agrawal; Rajat Varshney; Anil Gattani; P Kirthika; Mahvash Hira Khan; Rahul Singh; Shantanu Kodape; Shailesh Kumar Patel; Praveen Singh

doi:10.1016/j.mimet.2020.106024

Gold nanoparticle based immunochromatographic biosensor for rapid diagnosis of Mycobacterium avium subspecies paratuberculosis infection using recombinant protein

J Microbiol Methods. 2020 Oct:177:106024. doi: 10.1016/j.mimet.2020.106024. Epub 2020 Aug 12.

Authors

Aditya Agrawal¹, Rajat Varshney², Anil Gattani³, P Kirthika⁴, Mahvash Hira Khan⁵, Rahul Singh⁶, Shantanu Kodape⁷, Shailesh Kumar Patel⁸, Praveen Singh⁹

Affiliations

¹ Division of Animal Biochemistry, IVRI, Izatnagar, Bareilly, U.P. 243122, India. Electronic address: [email protected].
² Division of Bacteriology and Mycology, IVRI, Izatnagar, Bareilly, U.P. 243122, India; Department of Veterinary Microbiology, FVAS, IAS, RGSC, BHU, Barkachha, Mirzapur UP-231001, India. Electronic address: [email protected].
³ Division of Animal Biochemistry, IVRI, Izatnagar, Bareilly, U.P. 243122, India; Department of Veterinary biochemistry, Bihar veterinary college, Patna 800014, India. Electronic address: [email protected].
⁴ Division of Animal Biochemistry, IVRI, Izatnagar, Bareilly, U.P. 243122, India. Electronic address: [email protected].
⁵ Division of Animal Biochemistry, IVRI, Izatnagar, Bareilly, U.P. 243122, India. Electronic address: [email protected].
⁶ Division of Pathology, IVRI, Izatnagar, Bareilly, U.P. 243122, India. Electronic address: [email protected].
⁷ Division of Veterinary Biotechnology, IVRI, Izatnagar, Bareilly, U.P. 243122, India. Electronic address: [email protected].
⁸ Division of Pathology, IVRI, Izatnagar, Bareilly, U.P. 243122, India. Electronic address: [email protected].
⁹ Division of Animal Biochemistry, IVRI, Izatnagar, Bareilly, U.P. 243122, India. Electronic address: [email protected].

PMID: 32795639
DOI: 10.1016/j.mimet.2020.106024

Abstract

Highly infectious and obvious withstand ability of Mycobacterium avium subspecies paratuberculosis (MAP) to environment as well as lack of on-site field diagnostic methods notably hampers the paratuberculosis (PTB) control. The existing intricacy, time-consuming and complicated diagnostic methods of PTB accentuate the development of novel and easy-to-perform on-site test. A gold nanoparticle (GNP) based lateral-flow assay (LFA) using MAP recombinant protein (44 kDa) has been developed for sensitive and specific detection of PTB in field conditions. The diagnostic sensitivity and specificity of the LFA for MAP specific antibodies was found approximately 84.2% and 83.3% in comparison to indirect enzyme-linked immunosorbent assay. Consequently, the newly developed GNP based LFA offers on-site and cost-effective method for the prompt diagnosis of PTB and precludes the time-consuming laboratory screening.

Keywords: 44 kDa; Gold nanoparticles; Lateral flow assay; MAP; Paratuberculosis; Recombinant protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Bacterial
Bacteriological Techniques / methods
Biosensing Techniques / methods*
Chromatography, Affinity / methods*
Enzyme-Linked Immunosorbent Assay / methods
Gold / chemistry*
Metal Nanoparticles / chemistry*
Mycobacterium avium subsp. paratuberculosis / isolation & purification*
Mycobacterium avium-intracellulare Infection / diagnosis*
Mycobacterium avium-intracellulare Infection / microbiology
Recombinant Proteins*
Sensitivity and Specificity

Substances

Antibodies, Bacterial
Recombinant Proteins
Gold