Rapid identification of fungi from respiratory samples by Bruker Biotyper matrix-assisted laser desorption/ionisation time-of-flight using ID-FUNGI plates

Eur J Clin Microbiol Infect Dis. 2021 Feb;40(2):391-395. doi: 10.1007/s10096-020-04007-2. Epub 2020 Aug 17.

Abstract

Identification of moulds is crucial for the clinical management of patients. The goal of this study was to evaluate the new ID-FUNGI plate (IDFP) for the identification of moulds by MALDI Biotyper. IDFP was compared with Sabouraud with gentamicin and chloramphenicol plate (SAB) for the identification of 80 moulds from respiratory samples and eight reference strains. With the direct transfer method, species identification rose from 6% with SAB to 68% with IDFP using score cut-off 2 and from 20 to 75% using cut-off 1.7 (p < 0.001). Our study highlights that the new IDFP improves mycological diagnostic and workflow in laboratories.

Keywords: Bruker; Fungi; ID-FUNGI plate; MALDI TOF MS.

Publication types

  • Evaluation Study

MeSH terms

  • Fungi* / classification
  • Fungi* / isolation & purification
  • Humans
  • Lung Diseases, Fungal / diagnosis*
  • Mycological Typing Techniques / methods*
  • Point-of-Care Testing*
  • Respiratory System / microbiology*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*