Advanced chronic kidney disease is associated with higher serum concentration of monocyte microparticles

Life Sci. 2020 Nov 1:260:118295. doi: 10.1016/j.lfs.2020.118295. Epub 2020 Aug 18.

Abstract

Advanced chronic kidney disease is associated with high rates of cardiovascular disease. Considering the crucial role of capillaries in renal function, our study aimed to evaluate microparticles related to vascular physiology examining the link between stages of chronic kidney disease with circulating endothelial (EMP), platelet (PMP) and monocytic (MMP) microparticles. Cross-sectional study with blinded endpoints included subjects of both sexes, aged 40-75 years (n = 247), with established cardiovascular disease (coronary heart disease, ischemic stroke, or peripheral artery disease). They were stratified 1:1 by the presence or absence of decreased glomerular filtration rate (GFR < 60 mL/min/1.73 m2) estimated by the CKD-EPI criteria, and according to the stages of CKD. Microparticles were quantified by flow-cytometry using specific antibodies to identify endothelial, platelet, and monocytic derived microparticles. Higher percentages of circulating MMP (p = 0.036), but not for EMP or PMP, were observed in subjects with reduced GFR. Circulating MMP were also related to the stages of chronic kidney disease (trend analysis across renal stages, p = 0.038). Higher percentages of circulating MMP were found in subjects with reduced GFR, and their percentages were progressively higher according to the stage of chronic renal function.

Keywords: Cardiovascular disease; Chronic kidney disease; Glomerular filtration rate; Inflammation; Microparticles.

MeSH terms

  • Adult
  • Aged
  • Blood Platelets / ultrastructure
  • Cardiovascular Diseases / blood
  • Cell-Derived Microparticles*
  • Cross-Sectional Studies
  • Disease Progression
  • Endothelial Cells / ultrastructure
  • Female
  • Glomerular Filtration Rate
  • Humans
  • Male
  • Middle Aged
  • Monocytes / ultrastructure*
  • Renal Insufficiency, Chronic / blood*
  • Renal Insufficiency, Chronic / physiopathology