Strategies for 1 H-Detected Dynamic Nuclear Polarization Magic-Angle Spinning NMR Spectroscopy

Chemistry. 2020 Dec 4;26(68):15852-15854. doi: 10.1002/chem.202003463. Epub 2020 Nov 3.

Abstract

Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic-angle spinning NMR spectroscopy. Herein, the feasibility of proton-detected experiments with slow (10 kHz) magic angle spinning was demonstrated. The improvement in sensitivity permits the acquisition of indirectly detected 14 N NMR spectra allowing biomolecular structures to be characterized without recourse to isotope labelling. This provides a new tool for the structural characterization of environmental and medical samples, in which isotope labelling is frequently intractable.

Keywords: 14N NMR spectroscopy; amyloids; biomolecular NMR spectroscopy; dynamic nuclear polarization; solid-state NMR spectroscopy.