Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic-angle spinning NMR spectroscopy. Herein, the feasibility of proton-detected experiments with slow (10 kHz) magic angle spinning was demonstrated. The improvement in sensitivity permits the acquisition of indirectly detected 14 N NMR spectra allowing biomolecular structures to be characterized without recourse to isotope labelling. This provides a new tool for the structural characterization of environmental and medical samples, in which isotope labelling is frequently intractable.
Keywords: 14N NMR spectroscopy; amyloids; biomolecular NMR spectroscopy; dynamic nuclear polarization; solid-state NMR spectroscopy.
© 2020 The Authors. Published by Wiley-VCH GmbH.