Magnetic particle transport through organogel - an application to DNA extraction

Tetsuo Ohashi; Hiroki Kuyama

doi:10.1016/j.ab.2020.113932

Magnetic particle transport through organogel - an application to DNA extraction

Anal Biochem. 2020 Dec 15:611:113932. doi: 10.1016/j.ab.2020.113932. Epub 2020 Sep 4.

Authors

Tetsuo Ohashi¹, Hiroki Kuyama²

Affiliations

¹ Technology Research Laboratory, Shimadzu Corporation, 3-9-4 Hikaridai, Seika-cho, Soraku-gun, Kyoto, 619-0237, Japan. Electronic address: [email protected].
² Technology Research Laboratory, Shimadzu Corporation, 3-9-4 Hikaridai, Seika-cho, Soraku-gun, Kyoto, 619-0237, Japan.

PMID: 32891594
DOI: 10.1016/j.ab.2020.113932

Abstract

An observation that magnetic particles are transported through organogel encouraged us to investigate its feasibility of liquid-phase displacement in DNA extraction using magnetic particles. Organogel for this study was prepared from a gelator, 12-hydroxystearic acid (12-HSA), and an apolar solvent, methylphenylsilicone oil. The organogel is a gel-like solid material with hydrophobic and elastic properties. These properties, hydrophobicity, and elasticity were demonstrated to be advantageous for liquid compartmentalization and efficient liquid-phase displacement. The extracted DNA with using the organogel device was successfully detected off-chip by conventional real-time PCR.

Keywords: Immiscible phase; Magnetic particles; Nucleic acid extraction; Organogel; Sample preparation; Solid phase extraction.

MeSH terms

DNA / chemistry
DNA / isolation & purification*
Magnetic Phenomena*
Solid Phase Extraction*

Substances

DNA