A quick flow cytometry protocol to assess Helicobacter pylori viability

J Microbiol Methods. 2020 Oct:177:106043. doi: 10.1016/j.mimet.2020.106043. Epub 2020 Sep 5.

Abstract

Here we present an easy flow cytometry protocol to study the viability of Helicobacter pylori which also enables the detection of even low live bacteria densities. This protocol has potential utility for a fast and accurate assessment of experimental eradication methods against H. pylori.

Keywords: Flow cytometry; Helicobacter pylori; Propidium iodide; Syto 9; Viability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents
  • Bacteriological Techniques / methods*
  • Flow Cytometry / methods*
  • Helicobacter Infections / diagnosis*
  • Helicobacter Infections / microbiology
  • Helicobacter pylori / cytology
  • Helicobacter pylori / isolation & purification*
  • Humans
  • Microbial Viability
  • Organic Chemicals
  • Propidium

Substances

  • Anti-Bacterial Agents
  • Organic Chemicals
  • SYTO 9
  • Propidium