Characterization of the structure and analysis of the anti-oxidant effect of microalga Spirulina platensis polysaccharide on Caenorhabditis elegans mediated by modulating microRNAs and gut microbiota

Yihan Chen; Xuzhi Wan; Desheng Wu; Yuezhen Ouyang; Luying Gao; Zhengxin Chen; Hesham R El-Seedi; Ming-Fu Wang; Xinhua Chen; Chao Zhao

doi:10.1016/j.ijbiomac.2020.09.041

Characterization of the structure and analysis of the anti-oxidant effect of microalga Spirulina platensis polysaccharide on Caenorhabditis elegans mediated by modulating microRNAs and gut microbiota

Int J Biol Macromol. 2020 Nov 15:163:2295-2305. doi: 10.1016/j.ijbiomac.2020.09.041. Epub 2020 Sep 12.

Authors

Affiliations

¹ Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou, Fujian 350002, China; College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
² Department of Pediatrics, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
³ Division of Pharmacognosy, Department of Medicinal Chemistry, Uppsala University, Biomedical Centre, Box 574, SE-751 23 Uppsala, Sweden.
⁴ Food and Nutrition Department, Providence University, Taichung 43301, Taiwan.
⁵ Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
⁶ Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou, Fujian 350002, China; College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou 362000, China; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: [email protected].

PMID: 32931825
DOI: 10.1016/j.ijbiomac.2020.09.041

Abstract

The antioxidant activity of Spirulina platensis polysaccharide (SPP) was investigated in Caenorhabditis elegans. Nuclear magnetic resonance, Fourier-transform infrared, multi-angle laser light scattering, and GC-MS were used to identify the structural characteristics of SPP. It was composed of the →2)-α-L-Rhap-(1→, →4)-β-D-Manp-(1→, →6)-β-D-Glcp-(1→, →4)-β-Xylp-(1→, →3)-β-L-Araf-(1→, and →2)-β-L-Fucp-(1→, respectively. The modulation of gut microbiota in C. elegans was determined using 16S rRNA gene high-throughput sequencing. The malondialdehyde (MDA) content was significantly decreased, while the total superoxide dismutase (T-SOD) and reactive oxygen species (ROS) levels were improved after SPP supplementation. The cellular mitochondrial content and apoptosis were significantly down-regulated. The obvious increased levels of the DAF-16 and SKN-1 mRNAs were observed in the SPP-treated group, while the levels of miR-48 and miR-51 were significantly reduced. Moreover, SPP administration significantly increased the abundance of Flavobacterium, Achromobacter, Empedobacter, Anaerolinea, and Pseudoalteromonas of the intestinal flora. Based on these results, S. platensis polysaccharides may be used as a functional food to ameliorate diseases related to oxidative stress.

Keywords: Anti-oxidation; Caenorhabditis elegans; Gut microbiota; Spirulina platensis polysaccharide; microRNA.

MeSH terms

Animals
Antioxidants / chemistry
Antioxidants / pharmacology
Caenorhabditis elegans / drug effects
Caenorhabditis elegans / genetics
Gastrointestinal Microbiome / drug effects*
MicroRNAs / genetics*
Polysaccharides / chemistry
Polysaccharides / pharmacology*
Spirulina / chemistry*

Substances

Antioxidants
MicroRNAs
Polysaccharides

Supplementary concepts

Arthrospira platensis