Genome-wide identification and characterization of Respiratory Burst Oxidase Homolog genes in six Rosaceae species and an analysis of their effects on adventitious rooting in apple

PLoS One. 2020 Sep 25;15(9):e0239705. doi: 10.1371/journal.pone.0239705. eCollection 2020.

Abstract

Adventitious root formation is essential for plant propagation, development, and response to various stresses. Reactive oxygen species (ROS) are essential for adventitious root formation. However, information on Respiratory Burst Oxidase Homolog (RBOH), a key enzyme that catalyzes the production ROS, remains limited in woody plants. Here, a total of 44 RBOH genes were identified from six Rosaceae species (Malus domestica, Prunus avium, Prunus dulcis 'Texas', Rubus occidentalis, Fragaria vesca and Rosa chinensis), including ten from M. domestica. Their phylogenetic relationships, conserved motifs and gene structures were analyzed. Exogenous treatment with the RBOH protein inhibitor diphenyleneiodonium (DPI) completely inhibited adventitious root formation, whereas exogenous H2O2 treatment enhanced adventitious root formation. In addition, we found that ROS accumulated during adventitious root primordium inducing process. The expression levels of MdRBOH-H, MdRBOH-J, MdRBOH-A, MdRBOH-E1 and MdRBOH-K increased more than two-fold at days 3 or 9 after auxin treatment. In addition, cis-acting element analysis revealed that the MdRBOH-E1 promoter contained an auxin-responsive element and the MdRBOH-K promoter contained a meristem expression element. Based on the combined results from exogenous DPI and H2O2 treatment, spatiotemporal expression profiling, and cis-element analysis, MdRBOH-E1 and MdRBOH-K appear to be candidates for the control of adventitious rooting in apple.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Expression Regulation, Plant
  • NADPH Oxidases / genetics*
  • Plant Proteins / genetics*
  • Plant Roots / genetics*
  • Plant Roots / growth & development
  • Plant Roots / metabolism
  • Rosaceae / genetics*
  • Rosaceae / growth & development

Substances

  • Plant Proteins
  • NADPH Oxidases
  • superoxide-forming enzyme

Grants and funding

This research was funded by the National Natural Science Foundation of China (grant number 31801824), Specialist Project of Fruit Tree Innovation Team, Technical System of Modern Agricultural Industry in Shandong Province (SDAIT-06-05), Breeding Plan of Shandong Provincial Qingchuang Research Team (2019), and Scientific Research Funds for High-level Personnel of Qingdao Agricultural University (663/1118036 and 663/1119046). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.