A dual-analytes responsive fluorescent probe for discriminative detection of ClO- and N2H4 in living cells

Beitong Zhu; Xiaoli Wu; João Rodrigues; Xichao Hu; Ruilong Sheng; Guang-Ming Bao

doi:10.1016/j.saa.2020.118953

A dual-analytes responsive fluorescent probe for discriminative detection of ClO^- and N₂H₄ in living cells

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 5:246:118953. doi: 10.1016/j.saa.2020.118953. Epub 2020 Sep 17.

Authors

Beitong Zhu¹, Xiaoli Wu², João Rodrigues³, Xichao Hu⁴, Ruilong Sheng⁵, Guang-Ming Bao⁶

Affiliations

¹ School of Food and Drug, Luoyang Normal University, Luoyang 471934, PR China; Institute of Veterinary Drug, Jiangxi Agricultural University, Nanchang 330045, PR China.
² School of Food and Drug, Luoyang Normal University, Luoyang 471934, PR China.
³ CQM-Centro de Quimica da Madeira, Universidade da Madeira, Campus da Penteada, 9000-390 Funchal, Madeira, Portugal.
⁴ School of Food and Drug, Luoyang Normal University, Luoyang 471934, PR China. Electronic address: [email protected].
⁵ CQM-Centro de Quimica da Madeira, Universidade da Madeira, Campus da Penteada, 9000-390 Funchal, Madeira, Portugal. Electronic address: [email protected].
⁶ Institute of Veterinary Drug, Jiangxi Agricultural University, Nanchang 330045, PR China. Electronic address: [email protected].

PMID: 32987268
DOI: 10.1016/j.saa.2020.118953

Abstract

Hydrazine (N₂H₄) and ClO^- are very harmful for public health, hence it is important and necessary to monitor them in living cells. Herein, we rationally designed and synthesized a dual-analytes responsive fluorescent sensor PTMQ for distinguishing detection of N₂H₄ and ClO^-. PTMQ underwent N₂H₄-induced double bond cleavage, affording colorimetric and green fluorescence enhancement with good selectivity and a low detection limit (89nM). On the other hand, PTMQ underwent ClO^--induced sulfur oxidation and displayed red fluorescence lighting-up response towards ClO^- with good selectivity, rapid response (<0.2min) and a low detection limit (58nM). Moreover, PTMQ was successfully employed for in-situ imaging of N₂H₄ and ClO^- in living cells.

Keywords: Bioimaging; ClO(−); Dual-analytes; Fluorescent probe; Living cells; N(2)H(4).

MeSH terms

Colorimetry*
Fluorescent Dyes*
HeLa Cells
Humans
Limit of Detection
Spectrometry, Fluorescence

Substances

Fluorescent Dyes