SARS-CoV-2-derived peptides define heterologous and COVID-19-induced T cell recognition

Nat Immunol. 2021 Jan;22(1):74-85. doi: 10.1038/s41590-020-00808-x. Epub 2020 Sep 30.

Abstract

T cell immunity is central for the control of viral infections. To characterize T cell immunity, but also for the development of vaccines, identification of exact viral T cell epitopes is fundamental. Here we identify and characterize multiple dominant and subdominant SARS-CoV-2 HLA class I and HLA-DR peptides as potential T cell epitopes in COVID-19 convalescent and unexposed individuals. SARS-CoV-2-specific peptides enabled detection of post-infectious T cell immunity, even in seronegative convalescent individuals. Cross-reactive SARS-CoV-2 peptides revealed pre-existing T cell responses in 81% of unexposed individuals and validated similarity with common cold coronaviruses, providing a functional basis for heterologous immunity in SARS-CoV-2 infection. Diversity of SARS-CoV-2 T cell responses was associated with mild symptoms of COVID-19, providing evidence that immunity requires recognition of multiple epitopes. Together, the proposed SARS-CoV-2 T cell epitopes enable identification of heterologous and post-infectious T cell immunity and facilitate development of diagnostic, preventive and therapeutic measures for COVID-19.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • COVID-19 / immunology*
  • COVID-19 / prevention & control
  • COVID-19 / virology
  • Cross Reactions / immunology
  • Epitopes, T-Lymphocyte / immunology*
  • HLA-DR Antigens / immunology
  • HLA-DR Antigens / metabolism
  • Histocompatibility Antigens Class I / immunology
  • Histocompatibility Antigens Class I / metabolism
  • Humans
  • Immunologic Memory / immunology
  • Peptides / immunology*
  • SARS-CoV-2 / immunology*
  • SARS-CoV-2 / physiology
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / immunology*

Substances

  • Epitopes, T-Lymphocyte
  • HLA-DR Antigens
  • Histocompatibility Antigens Class I
  • Peptides
  • Viral Vaccines