LncRNA TUG1 promotes esophageal cancer development through regulating PLK1 expression by sponging miR-1294

Biotechnol Lett. 2020 Dec;42(12):2537-2549. doi: 10.1007/s10529-020-02984-0. Epub 2020 Oct 2.

Abstract

Objectives: Esophageal cancer is one of the malignant tumor with poor survival. The 5-year survival rate of esophageal cancer patients remains poor due to limited therapeutic options and the development of drug-resistance. Recent evidence suggests that long non-coding RNAs (lncRNAs) are involved in occurrence and development of tumor, however, the molecular mechanisms of lncRNA taurine-upregulated gene 1 (TUG1) in esophageal cancer remain unknown.

Results: TUG1 was overexpressed in esophageal cancer tissues and cells. The knockdown of TUG1 repressed proliferation and invasion, while promoted apoptosis of esophageal cancer cells by negatively regulating miR-1294 expression. Furthermore, PLK1 was a target mRNA of miR-1294 in esophageal cancer cells. Therefore, the effects of PLK1 silencing on proliferation, apoptosis, and invasion of esophageal cancer cells could be overturned by silencing miR-1294. Additionally, TUG1 silencing inhibited growth of tumor cells in vivo.

Conclusions: TUG1 was found as oncogenic gene in esophageal cancer. Mechanically, TUG1 attributed to esophageal cancer process by regulating miR-1294/ PLK1 axis.

Keywords: Esophageal cancer; LncRNA TUG1; PLK1; miR-1294.

MeSH terms

  • Apoptosis / genetics
  • Biomarkers, Tumor / genetics
  • Carcinogenesis / genetics
  • Cell Cycle Proteins / genetics*
  • Cell Line, Tumor
  • Cell Proliferation / genetics
  • Disease Progression
  • Esophageal Neoplasms / genetics*
  • Esophageal Neoplasms / pathology
  • Female
  • Gene Expression Regulation, Neoplastic / genetics
  • Humans
  • Male
  • MicroRNAs / genetics*
  • Polo-Like Kinase 1
  • Protein Serine-Threonine Kinases / genetics*
  • Proto-Oncogene Proteins / genetics*
  • RNA, Long Noncoding / genetics*
  • Transcriptional Activation / genetics

Substances

  • Biomarkers, Tumor
  • Cell Cycle Proteins
  • MIRN1294 microRNA, human
  • MicroRNAs
  • Proto-Oncogene Proteins
  • RNA, Long Noncoding
  • TUG1 long noncoding RNA, human
  • Protein Serine-Threonine Kinases