TAM and TIM receptors mRNA expression in Zika virus infected placentas

Guilherme M Nobrega; Ana Paula Samogim; Pierina L Parise; Emanuella M Venceslau; José Paulo S Guida; Rodolfo R Japecanga; Mariene R Amorim; Daniel A Toledo-Teixeira; Julia Forato; Sílvio R Consonni; Maria Laura Costa; José Luiz Proenca-Modena; Zika-Unicamp Network

doi:10.1016/j.placenta.2020.09.062

TAM and TIM receptors mRNA expression in Zika virus infected placentas

Placenta. 2020 Nov:101:204-207. doi: 10.1016/j.placenta.2020.09.062. Epub 2020 Sep 28.

Collaborators

Zika-Unicamp Network:
Eliana Amaral³, Helaine Maria Besteti Pires Mayer-Milanez³, Carolina C Ribeiro-do-Valle³, Roseli Calil³, João Renato Bennini Junior³, Giuliane Jesus Lajos³, Albina Altemani⁷, Maria Luiza Moretti⁸, Mariangela Ribeiro Resende⁸, Márcia Teixeira Garcia⁸, Rodrigo Nogueira Angerami⁸, Marcos Tadeu Nolasco da Silva⁹, Ana Carolina Coan¹⁰, Maria Francisca Colella-Santos¹¹, Andrea Paula Bruno von Zuben¹², André Ricardo Ribas Freitas¹², Marco Aurélio Ramirez Vinolo², Rodrigo Ramos Catharino¹³, Fábio Trindade Maranhão Costa², Clarice Weis Arns², Aline Vieira², Gabriela Fabiano de Souza², Karina Bispo Dos Santos², Mariene Ribeiro Amorim², Matheus Cavalheiro Martini², Stéfanie Primon Muraro²

Affiliations

¹ Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil; Department of Obstetrics and Gynecology, School of Medical Sciences, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.
² Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.
³ Department of Obstetrics and Gynecology, School of Medical Sciences, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.
⁴ Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.
⁵ Department of Obstetrics and Gynecology, School of Medical Sciences, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil. Electronic address: [email protected].
⁶ Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil; Experimental Medicine Research Cluster (EMRC), University of Campinas (UNICAMP), Campinas, São Paulo, Brazil. Electronic address: [email protected].
⁷ Department of Clinical Pathology, School of Medical Sciences, UNICAMP, Brazil.
⁸ Department of Internal Medicine, School of Medical Sciences, UNICAMP, Brazil.
⁹ Pediatric Immunology, Center for Investigation in Pediatrics, Faculty of Medical Sciences, Unicamp, Brazil.
¹⁰ Department of Neurology, School of Medical Sciences, UNICAMP, Brazil.
¹¹ Department of Human Development and Rehabilitation, School of Medical Sciences, UNICAMP, Brazil.
¹² Campinas Department of Public Health Surveillance, Campinas, Brazil.
¹³ School of Pharmaceutical Sciences, UNICAMP, Campinas, Brazil.

PMID: 33011564
DOI: 10.1016/j.placenta.2020.09.062

Abstract

To investigate the role of TYRO3, AXL and TIM1 receptors in the Zika virus (ZIKV) cycle, we determined their mRNA expression in different placental sites of ZIKV infected tissue during pregnancy. Unexpectedly, the ZIKV infection was not related with mRNA upregulation of these receptors or changes in expression of type I and III interferons in different placental sites. Instead, a decrease of TYRO3 mRNA expression was observed in positive sites of ZIKV positive placentas in comparison to negative sites. The basis of this downregulation can help to understand how ZIKV persists in placental tissue during pregnancy.

Keywords: Interferons; Placenta; TAM receptors; TIM receptors; Zika virus.; mRNA expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Axl Receptor Tyrosine Kinase
Case-Control Studies
Female
Hepatitis A Virus Cellular Receptor 1 / metabolism*
Host-Pathogen Interactions
Humans
Interferon Lambda
Interferon Type I / metabolism
Interferons / metabolism
Placenta / enzymology*
Placenta / immunology
Placenta / virology
Pregnancy
Pregnancy Complications, Infectious / immunology
Pregnancy Complications, Infectious / metabolism*
Pregnancy Complications, Infectious / virology
Proto-Oncogene Proteins / metabolism*
Receptor Protein-Tyrosine Kinases / metabolism*
Zika Virus / physiology
Zika Virus Infection / immunology
Zika Virus Infection / metabolism*

Substances

HAVCR1 protein, human
Hepatitis A Virus Cellular Receptor 1
Interferon Type I
Proto-Oncogene Proteins
Interferons
Receptor Protein-Tyrosine Kinases
TYRO3 protein, human
Interferon Lambda
Axl Receptor Tyrosine Kinase