By 2020, over 2.2 million people in the United States will be living with an amputated lower limb. The functional impact of amputations presents significant challenges in daily living activities. While significant work has been done to develop smart prosthetics, for the long-term development of effective and robust myoelectric control systems for transtibial amputees, there is still much that needs to be understood regarding how extrinsic muscles of the lower limb are utilized post-amputation. In this study, we examined muscle activity between the intact and residual limbs of three transtibial amputees with the aim of identifying differences in voluntary recruitment patterns during a bilateral motor task. We report that while there is variability across subjects, there are consistencies in the muscle recruitment patterns for the same functional movement between the intact and the residual limb within each subject. These results provide insights for how symmetric activation in residual muscles can be characterized and used to develop myoelectric control strategies for prosthetic devices in transtibial amputees.