The use of Gene-Xpert MTB RIF in the diagnosis of extrapulmonary tuberculosis in childhood and adolescence

Rev Soc Bras Med Trop. 2020 Oct 5:53:e20200104. doi: 10.1590/0037-8682-0104-2020. eCollection 2020.

Abstract

Introduction: Gene-Xpert MTB RIF (Xpert) is based on nucleic acid amplification by real-time polymerase chain reaction, which allows for the identification of Mycobacterium tuberculosis and rifampin resistance. We describe the use of Xpert for extrapulmonary tuberculosis (EPTB) in children and adolescents.

Methods: A case series of two reference centers in Rio de Janeiro from 2014-2019.

Results: The final diagnosis of EPTB was established in 11/36 (31%) patients, with five cases detectable by Xpert. For lymph node evaluation (9/11), diagnosis by Xpert occurred in 5/9 patients, all with caseous aspects.

Conclusions: Xpert can facilitate the rapid diagnosis of lymph node tuberculosis.

MeSH terms

  • Adolescent
  • Child
  • Child, Preschool
  • Humans
  • Infant
  • Mycobacterium tuberculosis*
  • Nucleic Acid Amplification Techniques
  • Rifampin
  • Tuberculosis*

Substances

  • Rifampin