Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening

Huyen Thi Lam Nguyen; Alice Soragni

doi:10.1016/j.xpro.2020.100056

Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening

STAR Protoc. 2020 Sep 18;1(2):100056. doi: 10.1016/j.xpro.2020.100056. Epub 2020 Jun 27.

Authors

Huyen Thi Lam Nguyen^{1

2}, Alice Soragni^{1

3

4

5

6}

Affiliations

¹ Department of Orthopaedic Surgery, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
² Technical Contact.
³ Molecular Biology Institute (MBI), University of California, Los Angeles, Los Angeles, CA, USA.
⁴ Jonsson Comprehensive Cancer Center (JCCC), University of California, Los Angeles, Los Angeles, CA, USA.
⁵ California NanoSystems Institute (CNSI), University of California, Los Angeles, Los Angeles, CA, USA.
⁶ Lead Contact.

Abstract

Tumor organoids are promising tools for cancer biology investigations and preclinical drug screenings because they are often representative of the histology and drug responses of patients. Here, we introduce a facile protocol to overcome technical limitations by generating patient-derived tumor organoids using a simplified ring-like geometry. This facilitates media exchange and drug treatment for histopathology characterization and automated high-throughput drug screenings. For complete details on the use and execution of this protocol, please refer to Phan et al. (2019).

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

High-Throughput Screening Assays / methods*
Histocytochemistry / methods*
Humans
Neoplasms* / chemistry
Neoplasms* / pathology
Organoids* / cytology
Organoids* / pathology
Tumor Cells, Cultured* / cytology
Tumor Cells, Cultured* / pathology

Abstract

Publication types

MeSH terms

Grants and funding