Laser spectroscopic technique for direct identification of a single virus I: FASTER CARS

Volker Deckert; Tanja Deckert-Gaudig; Dana Cialla-May; Jürgen Popp; Roland Zell; Stefanie Deinhard-Emmer; Alexei V Sokolov; Zhenhuan Yi; Marlan O Scully

doi:10.1073/pnas.2013169117

Laser spectroscopic technique for direct identification of a single virus I: FASTER CARS

Proc Natl Acad Sci U S A. 2020 Nov 10;117(45):27820-27824. doi: 10.1073/pnas.2013169117. Epub 2020 Oct 22.

Authors

Volker Deckert^{1

2

3

3}, Tanja Deckert-Gaudig^{2

3}, Dana Cialla-May^{2

3

3}, Jürgen Popp^{2

3

3}, Roland Zell⁴, Stefanie Deinhard-Emmer⁴, Alexei V Sokolov^{5

6}, Zhenhuan Yi⁵, Marlan O Scully^{1

7

6}

Affiliations

¹ Institute for Quantum Science and Engineering, Texas A&M University, College Station, TX 77843; [email protected] [email protected].
² Leibniz Institute of Photonic Technology, 07745 Jena, Germany.
³ Institute of Physical Chemistry, Friedrich-Schiller-Universität Jena, 07743 Jena, Germany.
⁴ Abbe Center of Photonics, Friedrich-Schiller-Universität Jena, 07743 Jena, Germany.
⁵ Institute for Quantum Science and Engineering, Texas A&M University, College Station, TX 77843.
⁶ Department of Physics, Baylor University, Waco, TX 76798.
⁷ Institute of Medical Microbiology, Jena University Hospital, 07747 Jena, Germany.

Abstract

From the famous 1918 H1N1 influenza to the present COVID-19 pandemic, the need for improved viral detection techniques is all too apparent. The aim of the present paper is to show that identification of individual virus particles in clinical sample materials quickly and reliably is near at hand. First of all, our team has developed techniques for identification of virions based on a modular atomic force microscopy (AFM). Furthermore, femtosecond adaptive spectroscopic techniques with enhanced resolution via coherent anti-Stokes Raman scattering (FASTER CARS) using tip-enhanced techniques markedly improves the sensitivity [M. O. Scully, et al, Proc. Natl. Acad. Sci. U.S.A. 99, 10994-11001 (2002)].

Keywords: coherent anti-Stokes Raman scattering (CARS); tip-enhanced Raman scattering (TERS); virus detection.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Lasers / standards
Limit of Detection
Microscopy, Atomic Force / instrumentation
Microscopy, Atomic Force / methods*
SARS-CoV-2 / ultrastructure*
Spectrum Analysis, Raman / instrumentation
Spectrum Analysis, Raman / methods*
Time
Virion / ultrastructure