Abstract
There is great interest in developing strategies to deliver proteins into the cytoplasm of cells. We report here a PEG-poly-eosin block copolymer (PEG-pEosin) that can encapsulate proteins and release them in active form under mildly acidic conditions. A PEG-pEosin formulation composed of Cre and the endosomolytic protein LLO efficiently performed gene editing in cells and in the brains of mice after an intracranial injection.
MeSH terms
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Animals
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Bacterial Toxins / antagonists & inhibitors*
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Bacterial Toxins / genetics
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Cell Survival / drug effects
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Eosine Yellowish-(YS) / chemistry
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Eosine Yellowish-(YS) / pharmacology*
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Erythrocytes / drug effects
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Gene Editing
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Heat-Shock Proteins / antagonists & inhibitors*
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Heat-Shock Proteins / genetics
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Hemolysin Proteins / antagonists & inhibitors*
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Hemolysin Proteins / genetics
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Hydrogen-Ion Concentration
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Mice
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Models, Molecular
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Molecular Structure
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Polyethylene Glycols / chemistry
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Polyethylene Glycols / pharmacology*
Substances
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Bacterial Toxins
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Heat-Shock Proteins
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Hemolysin Proteins
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Polyethylene Glycols
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hlyA protein, Listeria monocytogenes
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Eosine Yellowish-(YS)