CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids

Delilah Hendriks; Hans Clevers; Benedetta Artegiani

doi:10.1016/j.stem.2020.10.014

CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids

Cell Stem Cell. 2020 Nov 5;27(5):705-731. doi: 10.1016/j.stem.2020.10.014.

Authors

Delilah Hendriks¹, Hans Clevers², Benedetta Artegiani³

Affiliations

¹ Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences, and University Medical Center, Utrecht, the Netherlands; Oncode Institute, Utrecht, the Netherlands.
² Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences, and University Medical Center, Utrecht, the Netherlands; Oncode Institute, Utrecht, the Netherlands; The Princess Maxima Center for Pediatric Oncology, Utrecht, the Netherlands. Electronic address: [email protected].
³ The Princess Maxima Center for Pediatric Oncology, Utrecht, the Netherlands. Electronic address: [email protected].

PMID: 33157047
DOI: 10.1016/j.stem.2020.10.014

Abstract

CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

CRISPR-Cas Systems / genetics
Clustered Regularly Interspaced Short Palindromic Repeats* / genetics
Gene Editing
Genetic Engineering
Humans
Organoids*