Pulmonary infection due to fluoroquinolone-resistant Mycolicibacterium fortuitum: a case report

BMC Infect Dis. 2020 Nov 19;20(1):866. doi: 10.1186/s12879-020-05596-1.

Abstract

Background: Mycolicibacterium fortuitum is a species of the rapidly growing mycobacteria that can cause pulmonary infection. It is susceptible to multiple antibiotics both in vitro and in clinical practice, so that any combination of susceptible drugs is effective. However, we encountered a case of infection due to fluoroquinolone-resistant M. fortuitum. In this study, we report the case and describe the mechanism of resistance.

Case presentation: A 65-year-old man with a history of total gastrectomy and immunosuppressant treatment for rheumatoid arthritis developed a recurrence of pulmonary infection caused by M. fortuitum. He was treated with clarithromycin and levofloxacin as a first-line treatment, based on the favorable susceptibility at that time. After recurrence, a high minimum inhibitory concentration to fluoroquinolones was detected. DNA sequencing of the pathogen showed the substitution of serine for tryptophan at residue 83 in the gyrA gene. He was successfully treated with a combination of other antibiotics.

Conclusion: This is the first report on the treatment of fluoroquinolone-resistant M. fortuitum and investigation of the mechanism of resistance. We suggest that the susceptibility test remains effective for determining the next line of treatment after a pathogen has acquired resistance, and resistance to fluoroquinolones in M. fortuitum can be attributed to a single change of amino acid.

Keywords: DNA gyrase; Fluoroquinolone; Mycolicibacterium fortuitum; Resistance; gyrA.

Publication types

  • Case Reports

MeSH terms

  • Aged
  • Amino Acid Substitution
  • Anti-Bacterial Agents / pharmacology*
  • DNA Gyrase / chemistry
  • DNA Gyrase / genetics
  • DNA Gyrase / metabolism
  • Drug Resistance, Bacterial / genetics*
  • Fluoroquinolones / pharmacology*
  • Humans
  • Lung Diseases / diagnosis*
  • Lung Diseases / microbiology
  • Male
  • Microbial Sensitivity Tests
  • Mycobacterium Infections, Nontuberculous / diagnosis*
  • Mycobacterium Infections, Nontuberculous / microbiology
  • Mycobacterium fortuitum / drug effects*
  • Mycobacterium fortuitum / genetics
  • Mycobacterium fortuitum / isolation & purification
  • Recurrence
  • Sequence Analysis, DNA

Substances

  • Anti-Bacterial Agents
  • Fluoroquinolones
  • DNA Gyrase