High-Dimensional Phenotyping of Human Myeloid-Derived Suppressor Cells/Tumor-Associated Macrophages in Tissue by Mass Cytometry

Methods Mol Biol. 2021:2236:57-66. doi: 10.1007/978-1-0716-1060-2_6.

Abstract

Myeloid-derived suppressor cells (MDSCs) and tumor-associated macrophages (TAMs) are heterogeneous cells that share myeloid markers and are not easily distinguishable in human tumors due to their lack of specific markers. These cells are a major player in the tumor microenvironment and are involved in the prognosis and physiopathology of various tumors. Here is presented a scheme to decipher these cells by mass cytometry.

Keywords: Mass cytometry; Myeloid-derived suppressor cells; Tissue; Tumor-associated macrophages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / metabolism
  • Cell Membrane Permeability
  • Data Analysis
  • Flow Cytometry / methods*
  • Humans
  • Myeloid-Derived Suppressor Cells / pathology*
  • Phenotype
  • Staining and Labeling
  • Tumor-Associated Macrophages / pathology*

Substances

  • Antibodies