Crystal structure of the WD40 domain of human PLRG1

Xiaoyang Wang; Yanjun Li; Haiming Dai; Chao Xu

doi:10.1016/j.bbrc.2020.11.057

Crystal structure of the WD40 domain of human PLRG1

Biochem Biophys Res Commun. 2021 Jan 1:534:474-477. doi: 10.1016/j.bbrc.2020.11.057. Epub 2020 Nov 22.

Authors

Xiaoyang Wang¹, Yanjun Li², Haiming Dai³, Chao Xu⁴

Affiliations

¹ MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China.
² Structural Genomics Consortium, University of Toronto, 101 College St., Toronto, Ontario, M5G 1L7, Canada.
³ Anhui Province Key Laboratory of Medical Physics and Technology, Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China.
⁴ MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China. Electronic address: [email protected].

PMID: 33239170
DOI: 10.1016/j.bbrc.2020.11.057

Abstract

PLRG1 is a evolutionarily conserved protein in spliceosome and plays an important role in maintaining the integral part of the splicoeosme and its proper splicing. Here we solved the high resolution crystal structure of the WD40 domain of human PLRG1 by crystallography and compared our crystal structure with the cryo-EM structure of PLRG1 bound with other splicing factors. We found that two loops of the WD40 domain become resolved upon binding to the proteins within the spliceosome. Thus our work characterize the dynamic property of PLRG1 during the spliceosome assembly by presenting its apo structure.

Keywords: Spliceosome; The nineteen complex; WD40 repeats; X-ray crystallography.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crystallography, X-Ray
Humans
Intracellular Signaling Peptides and Proteins / chemistry*
Models, Molecular
Nuclear Proteins / chemistry*
Protein Domains
WD40 Repeats

Substances

Intracellular Signaling Peptides and Proteins
Nuclear Proteins
PLRG1 protein, human