NGS-based typings for 7 HLA loci in two populations from Barra Mansa, RJ, Brazil

Romulo Vianna; Leonardo Hanhoerderster; Juliana Cardoso; Luís Cristóvão Porto

doi:10.1016/j.humimm.2020.11.001

NGS-based typings for 7 HLA loci in two populations from Barra Mansa, RJ, Brazil

Hum Immunol. 2021 Jan;82(1):1-2. doi: 10.1016/j.humimm.2020.11.001. Epub 2020 Nov 27.

Authors

Romulo Vianna¹, Leonardo Hanhoerderster², Juliana Cardoso², Luís Cristóvão Porto²

Affiliations

¹ Laboratório de Histocompatibilidade e Criopreservação, Universidade do Estado do Rio de Janeiro, Avenida Marechal Rondon 381, 20950-003 Rio de Janeiro, Brazil. Electronic address: [email protected].
² Laboratório de Histocompatibilidade e Criopreservação, Universidade do Estado do Rio de Janeiro, Avenida Marechal Rondon 381, 20950-003 Rio de Janeiro, Brazil.

PMID: 33257011
DOI: 10.1016/j.humimm.2020.11.001

Abstract

We investigated HLA class I (HLA-A, -B, and -C) and class II (HLA-DRB1, -DQB1, -DPA1, and -DPB1) alleles by NGS-based typing among 478 Brazilian individuals from two populations in the Barra Mansa city based on their self-declared skin color (Caucasian, N = 405, AFND-ID: 3729; Black, N = 73, AFND-ID: 3731) to calculate allelic and haplotypic frequencies, plus linkage disequilibrium. No locus deviated from Hardy-Weinberg equilibrium. Both populations shared the most frequent allele on HLA-A, -C, -DPA1, and -DPB1. Genotype and frequency data are available in the Allele Frequencies Net Database.

Keywords: Barra Mansa; Brazil; HLA; Next-generation sequencing; Population immunogenetics.

MeSH terms

Alleles
Brazil
Gene Frequency
Genetics, Population / statistics & numerical data
Haplotypes
High-Throughput Nucleotide Sequencing
Histocompatibility Antigens Class I / genetics*
Histocompatibility Antigens Class II / genetics*
Humans
Linkage Disequilibrium
Population Groups / genetics

Substances

Histocompatibility Antigens Class I
Histocompatibility Antigens Class II